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PubMed Central, Liu, Feng, et al
GSH depletion causes ferroptosis in RPE cells To evaluate the mechanism of cell death in GSH-depleted cells, we treated RPE cells with various cell death pathway inhibitors including ferroptosis inhibitors ferrostatin-1 (Fer-1, 8 M), liproxstatin-1 (Lip-1, 600 nM), iron chelator deferoxamine (DFO, 80 M), pan-caspase inhibitor z-VAD-fmk (30 M) to inhibit apoptosis, autophagy inhibitor 3-methyladenine (3-MA, 10 mM), and lysosomal inhibitor bafilomycin A1 (Baf-A1, 75 nM)
A common beginner schedule is 100 mcg per shot, one shot per day at bedtime, on an empty stomach, for one week
Always consult your physician before starting any supplement regimen
The relative expression levels of each gene were quantitated using the 2 CT method and normalized to the amount of endogenous glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
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