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Resistance can be classified into intrinsic resistance, observed from the outset of the therapy, and acquired resistance, observed after an initial positive response to therapy [297]

doi: 10.1039/D5FB00127G 23

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The organic layers from two extractions were combined and aliquots were analyzed on a 4000 Q-Trap mass spectrometer (AB SCIEX, Framingham, MA) using the 50 2.1 mm Hydrophilic Interaction Liquid Chromatography (HILIC-LC) column (Waters Corp., Milford, MA)

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