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The mixture was vortexed for 20 s and placed in a 40 kHz Branson Ultrasonics bath for 30 min before adding 150 ng of MgSO 4 + 38 mg CH 3 COONa + 0.5 mL of DI water

GHK-Cu occurs naturally in human plasma at approximately 200 ng/mL at age 20, declining to about 80 ng/mL by age 60

Amino acids can be arranged in any order you like, so a chain of 15 amino acids has 20^15 possible sequences, or about 3.3 x 10^19, which is 33 quintillion possibilities

The wrong syringe size makes accurate dosing nearly impossible

Based on the rat study data, much lower doses (scaling from 0.5 micrograms per kilogram) may also be effective