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doi: 10.1126/science.1222939 107 SebastioAMColino-OliveiraMAssaife-LopesNDiasRBRibeiroJA

Substances prior to injection should be pure, sterile and warm (body temperature is ideal)

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Membranes were subsequently stripped and incubated with anti-SNAP tag rabbit polyclonal antibody (P9310S, New England Biolabs, 1:1,000) followed by goat anti-rabbit HRP secondary antibody (ab6721, Abcam, 1:2,000) to detect total level of immunoprecipitated SNAP/FLAG-hGLP-1R per sample