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For the cell number, cells were dissolved in phosphate-buffered saline (PBS, 140 mM sodium chloride, 10 mM disodium hydrogen phosphate, and 2.99 mM potassium dihydrogen phosphate (Carl Roth, Karlsruhe, Germany), pH 7.4)

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After the membranes were incubated in blocking buffer at room temperature for 2 h, they were washed 3 times in tris-buffered saline and tween 20 (TBST) solution, 5 min each, and then incubated with primary antibodies overnight at 4 C

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PMID: 3622582

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FFAR3, Free Fatty Acid Receptor 3

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This origin has a profound practical consequence: unlike nearly all other research peptides, the BPC-157 peptide is resistant to hydrolysis and enzymatic degradation in gastric conditions

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