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Samples were reduced with dithiothreitol at 10 mM for 30 minutes at 56C and alkylated with iodoacetamide at 30 mM for 45 minutes at room temperature in the dark

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Proc Natl Acad Sci U S A 1999

#4: Support Healthy Homocysteine and Reduce Oxidative Stress Work with a practitioner to optimize : Glutathione (via NAC or reduced glutathione)critical for maintaining RFC function Hydroxocobalamin (preferred B12 form)powerful nitric oxide scavenger that supports RFC function Folinic acid or Methylfolate (not folic acid) Vitamin B2 (riboflavin) and B6 (P5P form) TMG (trimethylglycine) #5: Avoid Green Tea Especially for: Pregnant women Children with neurological or developmental issues Anyone with diagnosed cerebral folate deficiency Avoid all forms: green tea beverages, matcha, green tea extract supplements, and products containing EGCG