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It is a 37-residue C-terminally amidated peptide closed by a Cys2-Cys7 disulfide bridge, sequence KCNTATCATQRLAEFLRHSSNNFGPILPPTNVGSNTP-NH, with the Lys1 side chain acylated by a C20 fatty diacid through a gamma-glutamate linker
200 UnissaA
Then, the supernatant containing the cell-free culture media was transferred to a new tube, and 50 mL of the Total Exosome Isolation reagent (Cat
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