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A genuinely stale or heat-damaged piece can feel dead. That said, the same manufacturer has a documented dissolution-failure recall on its record, so a minority of dead piece reports may be real release failures

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Fragments were cloned into the pCR-Blunt vector using the Zero Blunt PCR Cloning Kit (Invitrogen, Carlsbad, CA) and transformed into NEB 5-alpha competent E

A systematic review conducted by Callinan et al