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It should be noted that an NADH-bound Mt DHDPR structure showed a partially closed conformation, with the authors speculating that this unexpected closed structure might represent a snapshot of the equilibrium state between open and closed forms awaiting further substrate binding, and that subsequent substrate binding might induce the fully closed conformation 21

The plates were briefly stirred and activity was monitored at 418 nm in kinetic mode using a plate reader SpectraMax ABS Plus (Molecular Devices)

And I think it's, I don't think it's gonna take the next couple years or I hope not, because I think it's so important now, and I think it's something that's even overlooked, um, when it comes to what we're dealing with systemically is that's the first place we should look and sometimes it ends up being the last place that we look

The role of amifostine in the treatment of carcinoma of the uterine cervix: an update of RTOG-0116 and review of future directions