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Expression vectors (pFUSE2ss) encoding GLP-1 fused to the N-terminal end of albumin were constructed by sub-cloning cDNA fragments encoding two tandem GLP-1 peptide sequences (residues 736), both with the A2G substitution, in-frame of cDNA encoding human albumin variants (WT, LX, QMP, or QMP/LX) using the restriction enzyme sites EcoRI and Nhel (GenScript)