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Immune cell isolation from induced sputum and peripheral blood To eliminate mucus from sputum samples, the same volume of 0.1% dithiothreitol (Sigma, MO, USA) was added, the tube was shaken for 20 min at 37 C, and the mixture was filtered through a 70 m strainer

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This trial is only for people who are already interested in reducing or stopping their cannabis use if you have no issue with the amount of cannabis you are using, theres no reason for you to be involved