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Statements Author contributions RT: Writing original draft

Watanabe, S., Alexander, M., Misharin, A

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The fast-cycling mutant of hCdc42 (Cdc42/F28L) was generated by site-directed mutagenesis of hCdc42 in plasmid vector pGEX-2T (kindly provided by Dr Lars Helmsath, Max-Planck-Institute for Molecular Physiology, Dortmund) and subsequently subcloned into lentiviral vector pCDH-CMV-MCS-EF1-Puro