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In infants and children, breathing in secondhand smoke can: Cause children to develop asthma

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Each pack usually holds 20 cigarettes, making a carton contain a total of 200 cigarettes

GM-CSF and CXCL8 analysis by enzyme-linked immunosorbent assay The levels of GM-CSF, IL-1 and CXCL8 were measured in cell-free supernatants from PBECs cultures using the human CXCL8, IL-1 and GM-CSF DuoSet ELISA kit (R&D Systems, Minneapolis, MN) according to manufacturer's protocol