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The scientists found that seawater in the tubes with smoked filters had higher levels of metals than the unsmoked filters

Finally, the optical density (O.D.) of each well was measured within 10 min using a microplate reader set to 450 nm, and then the concentration was calculated by comparison with a standard curve

The SIRT plays a crucial role in protecting the cell against oxidative stress, maintaining and promoting DNA stability and regulating glucose and lipid metabolism thus having association with age-related pathologies (Kilic et al., 2015)

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