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PBS was used to gently resuspend the cells

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Studies have also indicated that elevated TNF- and dysregulated iron homeostasis often reinforce each other in tumor or inflammation models, collectively driving cell damage and death (120)

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Biomass quantification The cell debris collected after intracellular metabolite extraction was dried using a domestic microwave (250 W for 20 min) and weighed in order to estimate the total biomass content (dry weight) of each sample