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The cells were washed twice with DPBS Ca 2+ /Mg 2+ , and fresh RPMI phenol red-free media was added before live cell imaging

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NAD+ and mitochondrial biogenesis NAD+ works as a vital cofactor that changes metabolism, activates sirtuins, and keeps mitochondrial fitness through mechanisms like the mitochondrial unfolded protein response

Caspase 3 activity assay Fifty milligrams of frozen remnants livers were lysed in caspase 3 activity buffer (20 mM PIPES, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% CHAPS, 10% sucrose, pH 7.2)