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M., Forrest, P

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The high reactivity of GSH makes exact measurement of absolute concentrations challenging, likely leading to many discrepancies in reported concentrations in various biological systems

The starting reference models for each dataset were either the previously determined cryo-EM structure of PV3-SC8 from a plant expression system 18 (EMDB accession code EMD-3747) low-pass filtered to 60 to avoid bias for PV3-SC8 GPP3+GSH and PV3-SC8 pleconaril+GSH or for wt PV2-CP17 a 50 low-pass filtered model was generated from a prior reconstruction of a subset of the data

Selenium is a cofactor for the enzyme glutathione peroxidase, which plays a role in maintaining glutathione levels