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In addition, actin and Drebrin disulfide bond crosslinking, indicated by slower migration with smears in nonreducing western blots, was evident in glucose-deprived shGDH1 cells, which could be attenuated by TCEP (Supplementary Fig

2003 Jan;11(1):23-9

Peptides were injected onto a 75 m 2 cm trap column (Thermo Fisher) before re-focusing on an Aurora Elite 75 m 15 cm C18 column (IonOpticks) using a Vanquish Neo UHPLC (Thermo Fisher) connected to an Orbitrap Astral mass spectrometer (Thermo Fisher)