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Nat Rev Nephrol 22 , 137151 (2026)

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(2) based on the pseudoatomic model built obtained by AlphaFold and flexible docking into our cryo-EM map, which is further supported by computational membrane interaction prediction, we found that the AH of AtSNX1 has three negatively charged residues (D) and two positively charged residues (K and R) on its polar side, giving it a net negative charge (Fig