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Stable CHOFlpIn expression cell lines were selected using 600 g/ml hygromyocin B, and maintained in DMEM supplemented with 5% (V/V) FBS (Invitrogen) at 37C in 5% CO 2

Haqqani AS, Blanger K, Stanimirovic DB (2024) Receptor-mediated transcytosis for brain delivery of therapeutics: receptor classes and criteria